Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
Prostate
MeSH Description
A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.

Attributes by original data submitter

Sample

source_name
Normal Prostate
tissue
Normal Prostate
gleason score
-
tissue block size
5 x 4 mm
treatment
Untreated
antibody
AR Antibody - Spring Bioscience #E2724
ancestry
African

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Using a 2 mm2 core needle, one core was extracted from frozen RP tissue blocks in the areas marked on the corresponding slide. Frozen cores were pulverized using the Covaris CryoPrep system. DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Rubicon Genomics). Libraries were sequenced on an Illumina HiSeq 4000 to generate 150-bp paired-end reads (Novogene).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
25974372
Reads aligned (%)
77.7
Duplicates removed (%)
68.3
Number of peaks
10362 (qval < 1E-05)

hg19

Number of total reads
25974372
Reads aligned (%)
77.3
Duplicates removed (%)
69.1
Number of peaks
10306 (qval < 1E-05)

Base call quality data from DBCLS SRA