Antigen

Antigen Class

TFs and others

Antigen

AR

Cell type

Cell type Class

Prostate

Cell type

Prostate

MeSH Description

A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.

Sample

source_name

Normal Prostate

tissue

Normal Prostate

gleason score

-

tissue block size

5 x 4 mm

treatment

Untreated

antibody

AR Antibody - Spring Bioscience #E2724

ancestry

African

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Using a 2 mm2 core needle, one core was extracted from frozen RP tissue blocks in the areas marked on the corresponding slide. Frozen cores were pulverized using the Covaris CryoPrep system. DNA sequencing libraries were prepared using the ThruPLEX-FD Prep Kit (Rubicon Genomics). Libraries were sequenced on an Illumina HiSeq 4000 to generate 150-bp paired-end reads (Novogene).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

25974372

Reads aligned (%)

77.7

Duplicates removed (%)

68.3

Number of peaks

10362 (qval < 1E-05)

hg19

Number of total reads

25974372

Reads aligned (%)

77.3

Duplicates removed (%)

69.1

Number of peaks

10306 (qval < 1E-05)